Human Angiopoietin 2 (ANG-2) ELISA Kit

Cat.#:

JL10504

Regulatory Status:

RUO

Alternative:

ANG2; AGPT2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.078ng/mL

Specificity:

This kit recognizes Human ANG-2in samples. No significant cross-reactivity or interference between Human ANG-2 and analogues was observed.

Sample Type:

serum, plasma and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| ANG-2

Angiopoietin-2 is a protein that is encoded by the ANGPT2 gene.Naturally occurring antagonist for both ANGPT1 and TIE2; expressed only at the sites of vascular remodeling; similar to angiopoietin-1.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	0.23	1.79	1.27	0.8	0.51	0.32	0.22	0.12
Corrected OD	2.11	1.67	1.15	0.68	0.39	0.2	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human ANG-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Human ANG-2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98